In Vitro Growth Media Effect for Regeneration of Tomato (Lycopersicon esculentum) and Evaluation of the Salt Tolerance Activity of Callus

Lutfun Nahar Liza, A.N.M. Nasar, K.M.A. Zinnah, Md. Al Nayem Chowdhury, M. Ashrafuzzaman

Abstract


The identification of the best culture medium and the most suitable tomato variety was performed by In vitro regeneration. In the present study, attempts have been made to develop an efficient protocol for regeneration of V1 (ROMA VF), V2 (Baromasi Hybrid Tomato: JHOLOK) and V3 (Tomato Hybrid F1 JAGUR) varieties with their tolerance against abiotic stress (salt) to obtain stress tolerant tomato. The cotyledon induced in MS medium which was supplemented with T1 (MS+1mg/l BA+0.5mg/l NAA), T2 (MS + 1.5mg/l BA + 1mg/l NAA) and T3 (2mg/l BA + 1.5mg/l NAA) from seeds of those varieties. Maximum percentage (75%) of cotyledon induction was observed in T1 for V1, V2 and T3 for V3 respectively. Cotyledon derived from different concentrations of BA and NAA were cultured on MS medium supplemented with T3 (1.5 mg/l NAA, 2 mg/l BA) and TL (2.0 mg/l Kinetin) for plantlet regeneration. It was observed that MS media supplemented with T3 + TL produced lowest percentage of shoot (20%), callus (10%) and root (0%) in case of V2 and highest percentage of shoot (80%), callus(60%) and root (20%) in case of V3. Callus were transferred to the regeneration medium supplemented with NaCl (0 to 75mM) in order to examine their responses to salinity, the above three varieties, showed a significant decline in the callus growth. Present studies have shown that Hybrid F1 JAGUR variety is more responsive in regeneration and salt tolerant than others.

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