Expression of α-Amylase by Aspergillus flavus in Medium: Effect of Nitrogen Source of Growth

Adekunle Odunayo Adejuwon, Anthonia Olufunke Oluduro, Femi Kayode Agboola, Patrick Ojo Olutiola

Abstract


Aspergillus flavus is contaminant of storage products in the tropics. A defined medium with starch as carbon source and varied nitrogen sources was inoculated with spore suspensions of approximately 5x105 spores per ml of Aspergillus flavus. The nitrogen sources were ammonium chloride, urea, potassium nitrate, ammonium sulphate, glycine, sodium nitrate, tryptone and peptone. Extracellular α-amylase was produced by the fungus within a period of ten days in the inoculated defined medium. Peptone was able to induce highest α-amylase activity, expressed as 546 units/mg protein on the ninth day of inoculation of medium. Least activity were with tryptone and ammonium sulphate with optimum activities expressed as 5 units/mg protein on the eighth, ninth and tenth  days for tryptone and nil α-amylase activity on the tenth day when ammonium sulphate was nitrogen source.

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