Effect of Carbon Source of Growth on α-Amylase Production by a Strain of Penicillium funiculosum Thom. Isolated from Irish Potato (Solanum tuberosum Linn): Comparative Studies

Adekunle Odunayo Adejuwon, Olusola Abiola Ladokun

Abstract


Irish potato (Solanum tuberosum Linn.) is cultivated in the tropical middle belt of Nigeria. It is infested by a range of phytopathogens including Penicillium funiculosum Thom. In the present investigation, irish potato tubers and a defined growth medium were inoculated with pure culture of Penicillium funiculosum Thom. The composition of the defined medium was potassium dihydrogen sulphate, magnesium sulphate, calcium sulphate, hydrated iron sulphate, manganese sulphate, copper sulphate, zinc sulphate, thiamine, biotin, sodium nitrate as nitrogen source and varied carbon sources. Proteins which exhibited α-amylase activity were expressed and partially purified daily by ammonium sulphate fractionation followed by dialysis to remove small molecular weight impurities. The results showed that, optimum α-amylase activity was induced by the ninth day of inoculation of the potato tubers. Only traces of the α-amylase were observed in uninfected irish potato tubers. Similarly, starch, glucose, lactose, galactose, glucose and maltose as carbon source in the defined medium induced α-amylase activity varyingly. Highest value of induction was expressed when starch was carbon source followed by irish potato, then maltose glucose, sucrose, lactose, galactose consecutively. Earliest induction was with glucose with expression starting on the second day of inoculation of the defined growth medium. Inspite of being harmful to cultivation and storage of irish potatoes in Nigeria, West Africa, Penicillium funiculosum Thom. is a potential source of industrial production of α-amylase.

Full Text: PDF

Refbacks

  • There are currently no refbacks.


Copyright © 2018 InfinityPress.Info. All rights reserved.